The open reading frame of Pc-Hsp21 was 555 base pairs, encoding a protein of 184 amino acid residues with an alpha-crystallin family domain. Quantitative real-time PCR (qRT-PCR) analysis revealed a constitutive transcript expression of Pc-Hsp21 in the tested tissue, with the highest in hepatopancreas.

av G Rutsdottir · 2017 · Citerat av 1 — Combined structural methods to characterize an oligomeric small heat shock protein Hsp21. Rutsdottir, Gudrun LU (2017). Mark. Abstract: Small heat shock

2019-09-27 HSP21. Organism. Solanum lycopersicum (Tomato) (Lycopersicon esculentum) Status. Reviewed-Annotation score: -Experimental evidence at transcript level i. Function i Keywords i. Biological process: Stress response: Names & Taxonomy i.

Felsökning Allmänt .. HFP, HSP . 21. Ringe mens du spiller musikk .

A structure model of Hsp21, obtained by homology modeling, single-particle electron microscopy, and lysine-specific chemical crosslinking, shows that the Hsp21 subunits are arranged in two hexameric discs, rotated by 25 degree in relation to each other. HSP21 and pTAC5 formed a complex that was associated mainly with the PEP complex.

HSP21 was not detected in leaves or roots before stress, but the mature, 21-kD protein accumulated in direct proportion to temperature and HSP21 mRNA levels in both tissues. All of the HSP21 in leaves was localized to chloroplasts; there was no evidence for its transport into other organelles.

Our results suggest that HSP21 and pTAC5 are required for chloroplast development under heat stress by maintaining PEP function. HSP21 - Heat shock protein 21, chloroplastic precursor - Arabidopsis thaliana (Mouse-ear cress) - HSP21 gene & protein UniProtKB - P31170 (HS25P_ARATH) HSP21 - Small heat shock protein, chloroplastic precursor - Solanum lycopersicum (Tomato) - HSP21 gene & protein UniProtKB - Q95661 (HS21C_SOLLC) The open reading frame of Pc-Hsp21 was 555 base pairs, encoding a protein of 184 amino acid residues with an alpha-crystallin family domain. Quantitative real-time PCR (qRT-PCR) analysis revealed a constitutive transcript expression of Pc-Hsp21 in the tested tissue, with the highest in hepatopancreas.

2020-10-01

”spike”-proteinet hos HIV ska binda till CD4-. receptorn på cellmembranet har Rutsdottir: Combined structural methods to characterize an oligomeric small heat shock protein Hsp21. Kl 9.15 föreläsningssal B, Naturvetarevägen 14, Lund.

Structural information is lacking concerning the oligomeric conformation of this sHsp. We here present a structure model of Arabidopsis thaliana Hsp21, obtained by homology modeling, single-particle electron microscopy, and lysine-specific chemical crosslinking. De senaste tweetarna från @hsp21 Results are the mean ± SD of two independent experiments, each performed in duplicate with the length of at least 200 cells measured per strain and experiment.
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Mitt arbete gick ut på att undersöka Hsp21:s aktiva form och se om det förekom Hsp21-substrat komplex och i såfall vilken storlek de hade. Jag kunde observera två Hsp21-substrat komplex med två olika HSP21. Organism. Solanum lycopersicum (Tomato) (Lycopersicon esculentum) Status. Reviewed-Annotation score: -Experimental evidence at transcript level i.

Värd, Rabbit. Isotyp, IgG. Western blot, Yes. 24, 2018. The chloroplast‐localized small heat shock protein Hsp21 associates with the thylakoid membranes in heat‐stressed plants.
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HSP21 and pTAC5 formed a complex that was associated mainly with the PEP complex. HSP21 and pTAC5 were associated with the PEP complex not only during transcription initiation, but also during elongation and termination. Our results suggest that HSP21 and pTAC5 are required for chloroplast development under heat stress by maintaining PEP function.

Those interactions were detected by several methods such as yeast two hybrid, co-IP, pull-down and so on. We selected proteins and molecules interacted with HSP21 here. Most of them are supplied by our site. Hope this information will be useful for your research of HSP21.